Membrane Stability in Micrococcus Lysodeikticus and its Protoplasts
نویسندگان
چکیده
منابع مشابه
Membrane Fractions from Micrococcus lysodeikticus
1. A method is described for the isolation of pure mesosomal membrane fractions from Micrococcus lysodeikticus. 2. Plasmolysis of cells, before wall digestion, was necessary for effective mesosome release. 3. The effect of mild shearing forces, temperature and time upon the release of mesosomal membrane from protoplasts was investigated. 4. The optimum yield of mesosomal membranes from stable p...
متن کاملBinding of bacitracin to cells and protoplasts of Micrococcus lysodeikticus.
Bacitracin was tritiated by the Wilzbach technique and purified by carboxymethylcellulose chromatography. Binding studies between [3H]bacitracin and Micrococcus lysodeikticus cells and protoplasts indicated that the cells or protoplasts were saturated at 2 X lo5 molecules per cell. This value correlates well with the number of CS5-isoprenyl pyrophosphate molecules found in the membrane and adds...
متن کاملElectrochemical proton gradient in Micrococcus lysodeikticus cells and membrane vesicles.
Using the distribution of weak acids to measure the pH gradient (delta pH; interior alkaline) and the distribution of the lipophilic cation [3H]tetraphenylphosphonium+ to monitor the membrane potential (delta psi; interior negative), we studied the electrochemical gradient or protons (delta mu- H+) across the membrane of Micrococcus lysodeikticus cells and plasma membrane vesicles. With reduced...
متن کاملLiberation and osmotic properties of the protoplasts of Micrococcus lysodeikticus and Sarcina lutea.
Stable protoplasts may be released from MiCrococcua Zysodeikticus and Sarcina Zutea by digestion of the cell-wall with lysozyme in sucrose or NaCl solutions having an osmotic pressure of some 25 atmospheres, but not in glycerol solutions of the same osmotic pressure. The stability of the protoplasts depends not only upon the depression of the water activity by the solute but upon an osmotic pre...
متن کاملCharacterization of the membrane-bound succinic dehydrogenase of Micrococcus lysodeikticus.
The occurrence of succinic dehydrogenase [succinic:(acceptor) oxidoreductase, EC 1.3.99.1] in membrane fractions of Micrococcus lysodeikticus was investigated. The enzyme could be purified 10-fold, by deoxycholate treatment. Butanol extraction of membranes yielded an active fraction, nonsedimentable at 130,000 x g for 2 hr and altered in its phospholipid content relative to membranes. The activ...
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ژورنال
عنوان ژورنال: Australian Journal of Biological Sciences
سال: 1960
ISSN: 0004-9417
DOI: 10.1071/bi9600005